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PF-HT8 PRO

The fully automated multifunctional molecular detection instrument can analyze interactions between various biomolecules in real time, providing high-quality biological information on kinetics, affinity, antibody screening, and concentration determination without the need for labeling.

8-channel parallel detection, combined with fully automated ELISA detection, significantly improves kinetics/affinity screening throughput

Integrated temperature-controlled shaking incubation module from 4°C to 40°C, meeting the requirements for temperature-sensitive sample pretreatment and long-term incubation

Affinity testing for 8 samples in <10 minutes, kinetics screening for 96 samples in as fast as 5 minutes, supporting 12-hour real-time binding experiments

Baseline noise <0.3RU, drift <0.03RU/min, ensuring stable and reliable data

Supports various applications including kinetics, epitope identification, paired screening, and molecular fishing

Specifications

Detection channels: 8

Automation level: Fully automatic

Capacity: 6 x 96-well plates

Detection applications: Kinetics/Affinity characterization, Kinetics/Affinity screening, Small molecule interaction analysis, Fragment drug screening, Epitope identification, Paired screening, Molecular fishing

Sample incubation: 4°C-40°C with shaking incubation

ELISA detection: Fully automatic detection

Association constant (ka): Protein: 10¹~10⁷ M⁻¹·s⁻¹; Small molecule: 10³~5×10⁷ M⁻¹·s⁻¹

Dissociation constant (kd): 10⁻⁶~10⁻¹ s⁻¹

Affinity range (KD): 10 pM~1 mM

Lower detection limit: 150 Da

Detection time: Affinity testing for 8 samples in <10 min, up to 12h real-time kinetics binding experiments; kinetics screening for up to 96 samples simultaneously with minimum analysis time of 5 min, data sorted by binding signal intensity

Baseline noise (RMS): <0.3 RU

Baseline drift: <0.03 RU/min